The Primary Study on the Methods and Feasibility of Re-entry of NAT Reactive Blood Donors / 中国输血杂志

Objective To study the methods andfeasibility of re-entry of NAT reactive blood donors.Methods ELISA Non-Reactive(NR) and NAT Reactive(R) Blood Donors in 2012-2014 were selected and informed consent.Blood samples collected were tested:ELISA (HBsAg、Anti-HCV、Anti-HIV and Anti-TP) and NAT (HBV-DNA、HCV-RNA and HIVRNA).ELISA NR/NAT NR samples were tested by ECLA for HBsAg、HBeAg、Anti-HBs、Anti-HBc、Anti-HBe、Anti-HCV、Anti-HIV.Results 30 donors were called back,and 11 of them were ELISA NR/NAT R,which were deferred permanently.19 of them were ELISA NR/NAT NR,and the ECLA results were 89.5％ ELISA NR/NAT NR donors were Anti-HBc R and/or Anti-HBe R (12 cases Anti-HBc/Anti-HBe R、5 cases Anti-HBe R).Anti-HBc R or Anti-HBe R donors had risk of infection,which were deferred permanently.2 cases all items Non-Reactive may be eligible for re-entry.Conclusion Re-entry of NAT reactive blood donors is very necessary and meaningful,but in the view of blood safety,it is important that establish a set of safe program for Chinese.

Evaluation of the effects of methylene blue photochemical viral inactivation of red blood cells by using animal models / 中国输血杂志

Objective To evaluate the safety and effectis of methylene blue photochemical inactivation of virus in red blood cell (RBC) by using animal models. Methods ①The effects of improving anemia was assessed by transfusing MB P RBC to Beagle’s dog. ②Duck hepatitis B virus(DHBV)was serially diluted and added into human RBC, and then one day old ducklings were inoculated with that treated RBC by intravenous injection. Sera from ducklings were collected and tested by dot hybridization. Infectivity of DHBV (ID 50 ) in RBC was calculated before and after the treatment. Results Dog RBC treated by MB P were not hemolysed after autologous transfusion. There were no changes in serum ALT, AST, BUN, and creatinine after transfusion. The ID 50 contained in RBC changed from 10 3.35 to 10 8.35 after treatment. Conclusion MB P viral inactivation of RBC is safe and effective.

The Effect of Preserving Platelet Concentrate with Acidified Glucose Nutrient Solution / 中国输血杂志

The effect of preserving the platelet concentrate prepared from whole blood in domestic PVC plastic bags, using a new platelet-preserving addition——acidified glucose nutrient solution(AGN), in the condition of horizontal rotation at 22℃ was studied. The results of study showed that the platelet concentrate stored in the AGN solution for five days has its platelet counts, pH value, aggregability and hypotonic shock response (HSR) even more better than those of the control platelet concentrate preserved in the normal ACD solution. In addition, the platelet concentrate incubated with fresh plasma for two hours has its aggresability and hypotonic shock response obviously recovered. The observation of the platelet concentrate under transmission electron microscope indicated that the platelets preserved in AGN solution kept their morphology completed, without any pseudopodium. And so the effect of preserving the platelet concentrate with AGN solution in domestic PVC plastic bas is simiar to that obtained by foreign laboratories with CPD solution in the platelet preserving plastic bags of second generation.